Ferrocenylnaphthalene Diimide-based Electrochemical Ribonuclease Assay
نویسندگان
چکیده
منابع مشابه
Oral cancer diagnosis via a ferrocenylnaphthalene diimide-based electrochemical telomerase assay.
BACKGROUND Telomerase is regarded as a good marker for cancer because it is unregulated in most tumor cells compared with normal cells. We evaluated telomerase activity in the lysate of tumor tissue and surrounding cells of oral cancer patients by an electrochemical technique, dubbed the electrochemical telomerase assay (ECTA). METHODS The assay used ferrocenylnaphthalene diimide (FND) as the...
متن کاملDirect detection of single nucleotide polymorphism (SNP) with genomic DNA by the ferrocenylnaphthalene diimide-based electrochemical hybridization assay (FND-EHA).
A ferrocenylnaphthalene diimide-based electrochemical hybridization assay (FND-EHA) was applied to the direct detection of a C-to-G transition in a codon (TCA) for Ser-447 of the human lipoprotein lipase (LPL) gene, which resulted in the termination of the LPL protein there. Either one of two 13-meric oligonucleotide probes, S447 WT and S447X MT, representing sequences complementary to those of...
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A novel electrochemical assay to quantify transglutaminase activity is reported. The assay is based on the enzyme-controlled diffusion of Fe(CN)6(3-/4-) through amino-functionalized nanochannels of a mesoporous silica thin film on a Au surface in the presence of N-benzyloxycarbonyl-L-glutaminylglycine.
متن کاملDevelopment of a chemiluminescence-based ribonuclease protection assay.
The ribonuclease protection assay (RPA) is a widely used method for the detection and quantification of specific mRNA transcripts in a complex mixture of total RNA or mRNA molecules. While exhibiting many advantages over other RNA detection methods, RPAs are traditionally performed using radiolabeled probes that often require gel purification steps and lengthy exposure times to visualize result...
متن کاملCooperative binding of ferrocenylnaphthalene diimide carrying β-cyclodextrin converts double-stranded DNA to a rod-like structure.
Ferrocenylnaphthalene diimide carrying β-cyclodextrin (β-CD), 1, intercalated into double-stranded DNA with a binding affinity of K = (6.6 ± 0.8) × 10(4) M(-1) and a binding site size of n = 4, with a high positive cooperative parameter of ω = 14. β-CD and ferrocene moieties of the compound contributed to the formation of the intermolecular inclusion complex on DNA. Binding of 1 resulted in con...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2007
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.23.1415